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Because of the high amount of lipids in the brain, intense oxygen consumption and increased oxidative metabolism show us that the brain is sensitive to oxidative stress

Together, these findings highlight the long-standing clinical relevance of metabolic pathways targeted by antifolate therapy and underscore the importance of physiological folate forms, such as 5-methyl-THF, in modulating drug sensitivity and resistance

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All samples were analyzed on Agilent 1290 series HPLC system comprised of binary pumps, degasser and UV detector, equipped with an auto-sampler that is coupled with Agilent 6150 mass spectrometer