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Heidari-Khoei H, Esfandiari F, Moini A, Yari S, Saber M, Ghaffari Novin M, Piryaei A, Baharvand H (2022) Derivation of hormone-responsive human endometrial organoids and stromal cells from cryopreserved biopsies

In few instances the crude cell suspension is layered on a discontinuous gradient of 8.2% and 15.6 % (w/v) Nycodenz [14,15] but this gradient format is normally reserved for the purification of stellate cells and Kupffer cells

Finally, the gel was detained in 100 mL of destain solution (containing 8% acetic acid) by heating in the microwave (9501100 watts) for 45 seconds and shaking on an orbital shaker at room temperature until the desired background was achieved