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Other indirect ways to stimulate the antiepileptic effects of KD may lay in the alterations of the metabolism of neurotransmitters such as glutamate and gamma-amino butyric acid (GABA), and the activation of energy-sensing signaling pathways such as the PPAR, mammalian target of rapamycin (mTOR), and adenosine monophosphate-(AMP)-activated kinase (AMPK) pathways (71, 72)

doi: 10.1016/j.cell.2021.12.024 231 FrankDVinceJE

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Cell suspensions in PBS containing 0.5% BSA with a density of approximate 200 cells/l were used on the 10x Genomics Chromium platform to generate barcoded single-cell gel bead-in-emulsion (GEMs), targeting ~ 5000 cells per library