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Extracellular glutamine is taken up into neurons by SAT1,2 (Kanamori and Ross, Although not shown in Figure 3B (for the sake of simplicity), the subsequent de-amidation of glutamine to glutamate appears to be somewhat complex, probably reflecting the subcellular localization of the phosphate-activated glutaminase (PAG)

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For comparison, we treated cells with tunicamycin (TUN, a widely-used ER stress inducer) which did not alter phospho-GCN2 (T899), but reduced the mobility of PERK, indicative of its increased phosphorylation (Fig