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Even among healthcare practitioners, this super antioxidant remains underutilized in menopausal care

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For genotyping, DNA from toe clipping of littermate animals was extracted using a house-made Lysis buffer (0.1 M Tris-HCl pH 7.5, 0.1 M EDTA, 0.1 M NaCl, 1% SDS), and the PCR was performed using primers described in Supplementary Dataset 5 and the products were analyzed on an agarose gel

The reduction in these antioxidant enzymes (SOD, CAT, and GSH) leads to ROS accumulation, contributing to oxidative stress and cell viability loss