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13 One can increase t half_eff by increasing the amount of compound that partitions into tissue

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Using an equimolar amount of primer A (5-GTT CAG ACG TGT GCT CTT CCG ATC TNN NNN NNN NNN NNN NNT AGG TGT TGG GGC GGG ACT ATG GTT GCT GAC T-3), that anneals to the lesion-containing strand (123 nt downstream of the lesion) and contains a sample-specific barcode and 16 nt UMI sequence, a linear amplification was performed with Herculase II Fusion polymerase (1 unit, Agilent Technologies)

Ohanian M, Lin T, Ritchie EK, Craig M, Agrawal A, Halka KG, Karur V, Pemmaraju N, Borthakur G , Aminov S, Lim M, Pierce SA, Sacarakis N, Minor S, Ashizawa AT, Hahne W, Kantarjian H, Cortes JE, Roboz GJ