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Optimization Matrix: Conditions vs Selectivity The "Selective" column is the recommended starting point for any new construct, even before you know whether cryptic cleavage will occur

doi: 10.1016/j.compbiomed.2021.104790 169

Snchez-Villanueva et al., 2019)

In contrast, iron/oxygen receptors are similar to hypoxia-inducible factor prolyl hydroxylase and they are FBXL5 ubiquitin ligases that regulates IRP2 degradation