R&D Systems, Minneapolis, MN) in RPMI-1640 (Gibco, Detroit, MI) medium supplemented with 2% penicillin/streptomycin (Gibco), 20 mM HEPES (Gibco) and 5% pooled normal human heat-inactivated and sterile-filtered AB serum (Innovative Research, Novi, MI) for 7 days, with a media change on day 5 with fresh M-CSF (10 ng/mL)
In mouse colitis models, engaging Siglec-F (such as with a Siglec-F antibody) leads to eosinophil apoptosis and has been shown to reduce colonic eosinophil numbers, although the impact on inflammation severity varied by model (some acute colitis models didn't improve, whereas chronic models or asthma models clearly benefited from eosinophil suppression) (205208)
Besouw M, Blom H, Tangerman A, de Graaf-Hess A, Levtchenko E (2007) The origin of halitosis in cystinotic patients due to cysteamine treatment
Alveolar epithelial-derived exosomes are not merely products of barrier damage but key intercellular mediators (12), functioning through mechanisms that can drive the cascade diffusion of ferroptosis and bridge metabolic dysfunction with the immune storm (45) ( Figure 2 )