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It has been shown that roGFP2 is almost fully reduced in intact tissues and that a treatment with the GSH1 inhibitor BSO or the electrophilic xenobiotic 1-chloro-2,4-dinitrobenzene led to fully oxidized state of the biosensor accompanied by decreased fluorescence intensity 52

The slides were washed in PBS and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer