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Montagnese et al

In brief, using Fiji/ImageJ software, the raw image was split into three channels as an RGB stack, with the green channel selected for analysis due to its high contrast

Main transport proteins and metabolic events involved in bile-flow generation and in the hepatic handling of the endogenous organic anion, bilirubin (Br) and of the model exogenous cholephilic organic anion, sulphobromophthalein (BSP)