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Certain conjugation reactions form toxic reactive species (hepatotoxicity)

Bacterial pellets were resuspended and sonicated in lysis buffer (20 mM HEPES-KOH pH 7.5, 400 mM NaCl, 30 mM imidazole, 10% glycerol and 1 mM dithiothreitol) and purified using Ni-NTA resin (Qiagen, 30230)

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Development and validation of a machine learning-based diagnostic model for identifying nonneutropenic invasive pulmonary aspergillosis in suspected patients: A multicenter cohort study