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Sm-resistant colonies were streaked onto LB plates supplemented with 5% sucrose and grown at RT for 2 days to select for the second crossing-over event

MCC950 inhibits the activation of the NOD-like receptor protein 3, preventing inflammasome formation and pyroptosis [38]

In order to,bind the ligand on the surface of the support properly, protein A and G are usually used as a bridge which provides enough space for the ligand-protein binding