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Determining the Change in pH of TSB and 1 PBS as a Function of GSH Concentration Glutathione powder was weighed and directly dissolved into TSB (pH 7.18) and 1 PBS (137 mM NaCl, 2.7 mM KCl and 10 mM phosphate, pH 7.41) solution at room temperature under sterile conditions (bio-safety cabinet) to give 10, 20, and 30 mM of GSH

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Third, further research is needed to optimize dialysis modality-specific effects on eryptosis: while PD patients exhibit higher eryptosis levels than HD patients, potentially due to glucose-based dialysate components, conflicting data on RBC survival across modalities highlight the need for large-scale, longitudinal studies to compare the impact of conventional HD, hemodiafiltration (HDF), and biocompatible PD solutions on eryptosis rates and long-term anemia outcomes

Secondly, heterogeneous tissue distribution and low selectivity contribute to variable in vivo exposure