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The membrane was blocked with 5% skimmed milk in 100 mM Tris-HCl (pH 7.5), 150 mM NaCl and 0.2% Tween-20 (TBST) for 1 h at room temperature

doi: 10.1152/physrev.00012.2014

Ginsenoside Rh2-O suppresses hepatocellular carcinoma by inducing cell cycle arrest and apoptosis via activation of both intrinsic and extrinsic pathways