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Description

The Bradford method for protein quantitation

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436 Selinexor effectively impedes the nuclearcytoplasmic transport of the SARS-CoV-2 nuclear protein, thereby exerting inhibitory effects on viral proliferation

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Thus with the help of a glutathione assay, GSH/GSSG ratio is determined and the level of oxidative stress can be measured

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The prepared lysates were diluted 1:5 in an assay buffer and fluorescence intensities were measured using a Varioskan Flash spectral scanning multimode reader (Thermo Fisher Scientific, Inc.)

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