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Description

Journal of the American College of Surgeons 207(3):S102, 2008

Superoxide measurements in liver and kidney by electron paramagnetic resonance (EPR) spectroscopy Mitochondrial superoxide in liver and kidney tissue was measured by EPR spectroscopy using a mitochondrial targeted probe, 1-hydroxy-4-[2-triphenylphosphonio)-acetamido]-2,2,6,6-tetramethylpiperidine,1-Hydroxy-2,2,6,6-tetramethyl-4-[2-(triphenylphosphonio)acetamido] piperidinium dichloride (mito-TEMPO-H) (Enzo Life Sciences, Farmingdale, NY) 24,25

While the peak for GSH (2.98 min) gradually decreased, the signal corresponding to GSSG increased (4.37 min), indicative of the expected chemical transformation (Fig