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Description

Excess biotinylating agent was quenched and removed with three washes of PBS supplemented with 0.1 M glycine and cells were homogenized using a lysis buffer (150 mM NaCl, 20 mM Tris, and 1% Triton X-100, pH 7.5) containing protease inhibitors (Roche)

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how fMRI shows dehydration shrinks brain tissue and forces it to work harder for the same output (Kempton 2011)

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