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Briefly, 100 l of fresh mitochondria extracted from liver tissues were mixed with 900 l staining working solution and then the fluorescence intensity was assessed using a fluorescence microplate reader (BioTek, Winooski, VT, USA)

Tumor necrosis factor levels in control subjects compared to type 1 and type 2 diabetics A significant difference was demonstrated between the concentration of tumor necrosis factor in the study groups and the control group