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d , Representative FLIM images of polyGA inclusions in HEK293T cells expressing polyGA-SNAP at the indicated time points, labelled with 1 M S-SBD-SNAP

To determine whether the increase of LC3 puncta by the lysosomotropic compounds is due to upstream enhancement or downstream suppression of autophagy, we evaluated the change of abundance of SQSTM1/p62 with compound treatment