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10.1016/j.jcis.2017.11.064 8 CaoX.WangN

Western blots were performed as previously reported (17)

It is possible that the actions of endogenous opioids occur via an interaction with the GHS-R, as the original GH secretagogues characterized were derived from the enkephalins (336)

The flasks were then chilled at 4C for 1-2 hours before induction with 30 M IPTG (isopropyl -D-1-thiogalactopyranoside) and shaking at room temperature (RT), 180 rpm for 16-18 hours