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Briefly, a chilled methanol extraction of the whole cells or microsome fractions was freeze-dried and resuspended in 1% acetonitrile, and then applied to a Hydrosphere C18 column (YMC) on a Prominence nano HPLC system (Shimadzu, Kyoto, Japan) in line with a 4000 QTRAP mass spectrometer (AB Sciex Instruments, Foster City, CA) equipped with Hydrosphere C18 column (YMC)

Studies have consistently reported that inhibiting NNMT activity or downregulating NNMT expression leads to significant increases in NAD + levels in mouse adipocytes (Kraus et al., 2014

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