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They discovered a unique and specific manganese-binding site within the cytosolic cavity of the transporter that is formed by residues Asp40, Asn127, Asp248, and Ser252, and showed that the conserved residue Asp40 is critical, as its mutation abolishes Mn 2+ binding and transport

Sage et al., 2007)

10.1097/ico.0000000000002072 82 HiguchiA.InoueH.KanekoY.OonishiE.TsubotaK

A wide range of detergents have been employed for membrane protein solubilization [58-60] and, in the absence of any literature or laboratory precedent, the investigator will need to determine the best detergent for their particular protein empirically