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It was found that the OPFRs only increased the level of ROS and methemoglobin at very high concentrations: at 500 g/mL, they were found to induce morphological changes and ultimately cause lytic cell death and PS externalization

Depending on the structure and the number of electrons in the molecule, some RNS act as oxidising, nitrosating, or nitrating agents, which may react with various molecules, such as amines and thiols (Hughes 2008

The K d and IC 50 values were determined with the MO.Affinity Analysis software (NanoTemper Technologies GmbH), using two independent MST measurements

Recombinant tagged proteins are purified from cell lysates or supernatants by selective binding, followed by washing and elution of the purified protein