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Differential expression of histamine receptors in the bladder wall tissues of patients with bladder pain syndrome/interstitial cystitis significance in the responsiveness to antihistamine treatment and disease symptoms

The protein elution was concentrated with a 100-kDa cut-off concentrator (Millipore) and loaded onto a size-exclusion column (Superose 6 Increase 10/300 GL, Cytiva) equilibrated with buffer C containing 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM DTT, 0.06% digitonin (w/v)