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Proteins 72 , 10771083 (2008)

Proc Natl Acad Sci U S A 95:30716, PMID: 9501217 Won HY, Sohn JH, Min HJ, Lee K, Woo HA, Ho YS, Park JW, Rhee SG, Hwang ES (2010) Glutathione peroxidase 1 deficiency attenuates allergen-induced airway inflammation by suppressing Th2 and Th17 cell development

The formation of amide bond can be addressed using EDC only, however, the resulting O-acylisourea intermediate exhibits less stability in aqueous conditions: this reduces its reactivity toward amine moieties (due to hydrolysis) and the reaction yield