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To increase the retention duration in the lungs and allow them to pass through the mucus layer, nanoparticles can be made smaller, more electrically neutral, contain compounds that are specifically targeted, have a high encapsulation efficiency, and be less poisonous

Moreover, persistently high levels of blood glucocorticoids can lead to the mobilisation of energy stores (liver, fat and muscle), inflammation, neuronal death and even exacerbate depression [11]

Coupled in vitro transcription and translation reactions were used to generate SMN and delta-Tudor SMN proteins with 35 S-methione and cysteine (Promega)

Next, to assess basal startle responding, animals were exposed to startle tones of increasing intensity (65115, 5 dB increment, 60 s interval)