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To block nonspecific binding and permeabilize the cells, samples were incubated in blocking buffer (5% bovine serum albumin (BSA) and 0.3% Triton X-100 in PBS) for 1 h at room temperature

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14 performed measurements at 72 h, 14 days, and 30 days post-injury, and, consistent with our results, found no alterations to tNAA over time

Electrosprayed nanoparticles and electrospun nanofibers based on natural materials: applications in tissue regeneration, drug delivery and pharmaceuticals