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The LAT1 amino acid transporter system coordinates with mTOR activation to drive metabolic reprogramming

For polar extraction, 180l of 40:40:20 acetonitrile:methanol:water was added to each frozen cell pellet on dry ice

and (iv) redox exchange reactions occurring between the low-molecular-weight thiols and cysteines of plasma-membrane bound proteins, acting as redox switches

siRNA-mediated dynamin knockdown siRNA transfection The neurons were co-transfected with 50 nM dynamin 1, 2, and 3 siRNA constructs (previously described in 65 ) or with non-targeting control siRNA (ON-TARGETplus Non-targeting Control Pool