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The fresh mass (FW) of pooled discs per replicate were determined immediately

At the microbiological level, structural remodeling and functional abnormalities of the intestinal microbiota have been identified as key triggers for both initiation and maintenance of inflammation through metabolic interactions and immune regulatory pathways [24,25,26,27,28]

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R&D Systems, Minneapolis, MN, USA) and treated by SP (2.5 10 6 cells/mL) or MTX (4 M) for 0, 1, 3, and 5 days