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The top 5 proteins that were up-regulated most in expression after riboflavin deficiency were H31, RPS29, AK1C2, GSH0 and TRXR1, whereas AOFB, DLD, NQO1, NDUFS1 and DDX11 were the top 5 proteins that were down-regulated most (Table 1)

2.15 Competitive ligand binding studies of HPX with heparin To assess the relative affinities of HPX for heparin and heme, aliquots of heparin were added to HPX in phosphate-buffered saline (4 C), 45 min before mesoheme