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The analysis used the Hwang-Shih-De Cani alpha spending function with parameter gamma = 4 (OBrien-Fleminglike) for the upper (superiority) bound under the null hypothesis with total one-sided Type I error 2.5%, and for the lower (safety or futility) bound under the alternative hypothesis with total Type II error 20% (80% power)

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in 1972 established that the catalytic activity of GPx depended on selenium status of the erythrocytes [3]

Thus, as the SLC1A5 variant is the only currently known glutamine transporter in the mitochondrial inner membrane 16 , targeting the SLC1A5 variant could be an effective strategy for selectively inhibiting glutamine metabolism in cancer cells (Fig