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K-562 cells were transfected using the Lipofectamine 2000 Reagent (11,668019, Invitrogen) following the manufacturers instructions

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The IV delivery ensures maximum absorption and faster results compared to other methods

At specific time points (0, 4, 8, 24 and 48 hours), 1.0 mL of nanoparticle suspension was centrifuged at 19354 g for 10 minutes (Centurion Scientific Ltd., UK)