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doi: 10.3389/fimmu.2024.1447213 58 ZhangFJonssonAHNathanAMillardNCurtisMXiaoQet al

As an unsupervised statistical method, PCA can simplify the multivariate data into a few principal components, which can highlight intrinsic trends, distribution of clusters and the existence of outliers

After treatment, cells were washed, tripsinized and resuspended in FACS buffer (PBS with 5% FBS and 1mM EDTA) and analyzed on an LSR Fortessa (BD Biosciences) with plate reader operated on BD FACSDiva software (v9.0)