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Individual clones containing LacAnc100 and LaccID were inoculated into 50 l of SC minimal medium containing 6.7 g l 1 yeast nitrogen base (BD, 291940), 1.92 g l 1 yeast synthetic dropout medium supplement without uracil (Merck, Y1501), 20 g l 1 d-raffinose (Merck, R0250) and 25 g ml 1 chloramphenicol (Merck, C0378) in a sterile 96-well plate (Enzyscreen, CR1496c)

I'm sure these journalists aren't sitting there watching this Senate hearing going, you know what

[23] Gustav married Aurelia on October 20, 1945

The surface was washed between samples with 25 mM NaOH for 4 s followed by equilibration with running buffer