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The cultures were allowed to grow for 5 days, washed with PBST, and resuspended in a final volume of 1 ml PBST at density of OD 0.5

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The passive pressure-diameter relationships in middle cerebral arteries that were maximally dilated with Ca 2+ free solution were similar in wild type and Nrf2 (/) knockout rats fed LS diet or HS diet ( Protandim) (Supplement Figure 3), indicating that NRF2 activation with Protandim does not affect the passive mechanical properties or distensibility of cerebral arteries of HS-fed wild type or Nrf2 (/) knockout rats (Supplement Figure 3)

As estrogen declines, so does its protective effect on the nervous system, which can lead to increased inflammation, worsened fatigue, and a potential acceleration of disease progression