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Due to technical advances in the visualization of glutathione and the redox state through microscopical methods some progress was made in the last few years in studying the importance of subcellular glutathione contents during stress conditions in plants

To obtain ratiometric images, the masks were applied to the background-subtracted images and the FP channel was divided by the SiR channel in a pixel-by-pixel manner within the ROIs

Final model performance was evaluated on the hold-out set and assessed by fold-improvement over incidence of AATD-LD observed in the IQVIA AEMR data

Using MRM analysis, we found that GF caecum samples contained 30-fold less indole-3-acetate compared with SPF samples (Supplementary Fig