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Ethion, chlorpyrifos, diazinon, cypermethrin and amitraz were purchased from Wako Pure Chemical Industries, Ltd

Cytochrome c release assay Cells were fractionated to obtain cytoplasmic and mitochondria-enriched fractions using Dounce homogenization and differential centrifugation as per the Abcam cytochrome c release kit (ab65311)

It is harmless and unlikely to repeat itself for the majority of shots unless the shots are always being given at the most extreme angle, almost horizontal in nature