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As a cofactor, cobalamins are essential for two cellular reactions: the mitochondrial methylmalonyl-CoA mutase conversion of methylmalonic acid (MMA) to succinate, which links lipid and carbohydrate metabolism, and the activation of methionine synthase, which is the rate-limiting step in the synthesis of methionine from homocysteine and 5-methyltetrahydrofolate

Interestingly, RIPA-56 and Necrostatin-1 (Nec-1, RIPK1 inhibitor) were confirmed to inhibit necroptosis and improve the inflammatory signaling pathway activated in mice fed high-fat diet (Majdi et al., 2020

On this basis, MSD21, MSD24, and MSD37 were selected as the most suitable producers for detailed postbiotic characterization, whereas MSD74, ML57, and MSD108 were retained as indicator strains for assessing the prebiotic activity of the resulting postbiotics

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