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Briefly, stepwise transient dominant selection to produce EEHV-MVA vector was performed as follows: The B8 shuttle vector 77 containing EE2 was recombined in DF-1 cells (American Type Culture Collection, Manassas, VA, USA) with a parental virus, MVA-F11 mCherry B8-BFP, which expresses two reporter genes, mCherry driven from the F11 promoter and blue fluorescent protein (BFP) driven from the B8 promoter

After incubation with SAC (1 M or 100 M), TMZ (500 M) or TMZ + SAC at different concentrations, media were aspired, and cells were homogenized in lysis buffer

Table 1: Important considerations to avoid feline injection-site sarcoma (FISS) development Acknowledgement ABCD Europe gratefully acknowledges the support of Boehringer Ingelheim (the founding sponsor of the ABCD), MSD Animal Health, Vtoquinol and Virbac