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Some practitioners combine both approaches

doi: 10.1038/nature12726 220 FurusawaYObataYFukudaSEndoTANakatoGTakahashiDet al

For example, in the peroxisome where verylongchain FAs are preferentially metabolized, carnitine shuttles the fatty acyl end products of beta oxidation between the peroxisome and the mitochondria (Steiber et al., 2004)

To compare simulated concentration time-courses with measured time-course data, we include the unknown concentration scale of fluorescence microscopy measurements and possible background fluorescence into an observation function