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Moreover, MTX-treated animals (group 4) demonstrated moderate records of tubular degeneration with significant loss of luminal border integrity of proximal and distal convoluted tubules (red arrows) with occasional luminal detached epithelial cells (black arrow), marked congested and dilatation of glomerular tuft capillaries (black arrow head), mild periglomerular and interstitial mononuclear inflammatory cells infiltrates were observed (red arrow head) accompanied with mild interstitial and perivascular fibrosis in Massons trichrome-stained sections