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Purification of GST-tagged proteins For protein expression, 400 mL bacterial cultures (DH5 or BL21 Codon Plus RIPL) were transformed with the corresponding expression plasmids, grown in LB medium supplemented with appropriate antibiotics and induced with 0.1 mM IPTG at OD 600 = 0.81.0

these microbial metabolites, as butyrate, can act as signals to peripheral clocks and help maintain rhythm synchrony

(19) Cystic fibrosis leads to suppressed glutathione levels and the impaired ability to produce more at a normal rate

It facilitates cell migration to injury sites, promotes cell differentiation, supports actin-mediated tissue remodeling, and reduces scar tissue formation