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Description

In the days prior to 17 March 2017, ISIS fighters conducted complex attacks against the CTS FLOT

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Cell pellets were lysed in lysis buffer (50 mM Tris-Cl pH 8, 150 mM NaCl, 1% Triton, 1x protease inhibitors, 1 mM PMSF, 2 mM NaF, 50 units/mL benzonase and 1 mM DTT) as described under Immunoprecipitation