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Hepatitis C virus (HCV) proteins induce NADPH oxidase 4 expression in a transforming growth factor beta-dependent manner: a new contributor to HCV-induced oxidative stress

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RNA-seq and qPCR analysis confirmed that CD90 + PDGFRA + cells expressed portal fibroblast markers (for example, DCN and THY1 ), some VSMC markers ( MYH11 ) and several growth factors ( HGF and WNT5A , among others), all enriched in portal mesenchyme in vivo, and were negative for markers of other liver stromal populations, such as hepatic stellate cells (for example, LRAT ) and mesothelia (for example, OGN ) (Extended Data Fig