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The nuclear pellet was washed and then lysed in buffer C (20 mM HEPES, pH 7.9, 200 mM NaCl, 1 mM EDTA, 5% v v 1 glycerol, 1 mM DTT, 1 mM Na 3 VO 4 , 1 mM PMSF, and 10 mg mL 1 aprotinin and leupeptin)

A second meaning is related to the preservation of memory and local identities

The progression of TBI typically entails two phases: the initial primary mechanical injury and subsequent delayed secondary injury (Kaur and Sharma, 2018)