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Multiple colon tumour areas were assessed per animal, and the average percentage of CC3 + cells per tumour is shown

RNA isolation and quantitative RT-PCR Total RNA from cells was isolated with Trizol reagent (Qiagen GmbH, Hilden, Germany) and RT-PCR was conducted using a quantitative SYBR Green RT-PCR kit (Applied Biosystems, Warrington, UK) was used with a Step One Plus RT-PCR System (Applied Biosystems)

Specifically, we observed that gene expression level of AMER 3, NRARP , CCND1 , LRG1 , and LEF1 were increased 5.2-fold, 2.6-fold, 2.1-fold, 2.2-fold, and 2-fold, respectively, at output voltage of 4.2 kV, whereas only CCND1 and LEF1 were increased 2-fold, each, at output voltage of 5.0 kV

Conclusion Refrigerating this liposomal glutathione helps maintain its potency and extends shelf life