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3) the L-carnitine standard solution and the D-carnitine standard solution of step 2) gained are respectively made into the L-carnitine derivative solution of the gradient concentration and the D-carnitine derivative solution of the gradient concentration

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However, the BHMT reaction is limited to the human liver and kidney since BHMT is expressed primarily in the hepatocytes and kidney cortex (Sunden et al., 1997)