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Next, we tested the robustness of TGP for FSEC-TS assay using the ultra-stable PlsY from Aerocus aquifex ( aa PlsY, 20.9 kDa, 7 transmembrane helices), which has a half-life of 30 min at 90 C as revealed by an activity-based assay 65

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While trivalent Mn can bind to transferrin, studies where rat brains were perfused with Mn solutions and experiments measuring Mn cellular uptake have shown that Mn 3+ -transferrin is taken up much more slowly than Mn 2+ species [135, 136], suggesting that TfR1 might not be a critical Mn carrier mediating Mn influx across the BBB