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Fe 3+ is released from LCN2 and may be reduced to Fe 2+ and transported to the cytoplasm, enabling efficient iron recycling by the cell

In vitro studies have shown that Tangshenning reduces HG-induced podocyte injury by inhibiting the mTORC1 pathway (including p-mTOR, mTOR, p-p70S6K, p70S6K, ULK1, and 4EBP1) and its downstream targets, while restoring podocyte autophagy (Xu et al., 2022)

For instance, it has been shown that after an experimental psychologic stress, the evoked cortisol response in fatigued breast cancer survivors is much smaller than that in nonfatigued ones, pointing to the involvement of the HPA axis in cancer-related fatigue (286)

Cercarial pellets were resuspended and washed thrice in serum-free M199 medium and collected at 100 g for 5 min