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Sample mounting, staining and imaging: ultra-thin sections (85 nm) obtained with a Leica Ultracut UCT ultramicrotome (Leica Microsystems) were mounted on copper grids with parlodion-carbon support film, placed sideways on a droplet of 2% uranyl acetate in H 2 O for 30 min, immersed repeatedly in H 2 O, air dried overnight, placed sideways on a droplet of Satos lead staining solution for 20 min, immersed repeatedly in H 2 O, air dried for 30 min and imaged at 80 kV and 50,000 magnification

Too conservative, and results disappoint

Matching the diluent to how the solution will be handled is a basic part of sound laboratory practice

Most start at 1.5-2.5 mg total peptide per injection